|
|
TaKaRa LA Taq™ |
|
TaKaRa Taq、TaKaRa Ex Taq、TaKaRa LA Taq的扩增实验例 |
|
|
扩增λ DNA |
|
扩增人基因组DNA |
TaKaRa Taq |
 |
|
 |
目的片段大?。骸?0 kb |
|
目的片段大?。骸? kb |
|
TaKaRa Ex Taq |
 |
|
 |
目的片段大?。骸?0 kb |
|
目的片段大?。骸?0 kb |
|
TaKaRa LA Taq |
 |
|
 |
目的片段大?。骸?0 kb |
|
目的片段大?。骸?0 kb |
|
|
扩增λDNA |
|
扩增人基因组DNA |
|
Lane A: |
pHY Marker |
Lane 7: |
10 kb |
|
Lane A: |
pHY Marker |
Lane 7: |
28.4 kb |
|
Lane 1: |
0.5 kb |
Lane 8: |
12 kb |
|
Lane 1: |
0.262 kb |
Lane 8: |
29.9 kb |
|
Lane 2: |
1 kb |
Lane 9: |
15 kb |
|
Lane 2: |
2.9 kb |
Lane 9: |
30.8 kb |
|
Lane 3: |
2 kb |
Lane 10: |
20 kb |
|
Lane 3: |
8.5 kb |
Lane A: |
pHY Marker |
|
Lane 4: |
4 kb |
Lane 11: |
28 kb |
|
Lane 4: |
17.5 kb |
Lane B: |
λ-Hind III Marker |
|
Lane 5: |
6 kb |
Lane 12: |
35 kb |
|
Lane 5: |
23.2 kb |
|
|
|
Lane 6: |
8 kb |
Lane B: |
λ-Hind III Marker |
|
Lane 6: |
27 kb |
|
|
|
|
|
【PCR条件】 |
● 扩增λDNA |
Lane 1-3: |
94℃, 1min. |
|
|
|
Lane 4-12: |
94℃, 1min. |
|
|
|
98℃, 5 sec. |
 |
30 cycles |
98℃, 5 sec. |
 |
30 cycles |
68℃, 5 min. |
68℃, 15 min. |
72℃, 10 min. |
|
|
72℃, 10 min. |
|
|
|
|
● 扩增人基因组DNA |
Lane 1: |
94℃, 1 min. |
|
|
|
Lane 2: |
94℃, 1 min. |
|
|
|
Lane 3-9: |
94℃, 1min. |
|
|
|
94℃, 30 sec. |
 |
30 cycles |
94℃, 30 sec. |
 |
30 cycles |
58℃, 1 min. |
|
58℃, 1 min. |
|
98℃, 5 sec. |
 |
30 cycles |
|
72℃, 1 min. |
|
72℃, 3 min. |
|
68℃, 15 min. |
|
72℃, 10 min. |
|
|
|
72℃, 10 min. |
|
|
|
72℃, 10 min. |
|
|
|
|
|
|